Difference Between HPLC and Fast HPLC

March 2022 · 3 minute read

HPLC is high performance liquid chromatography and fast HPLC is an advanced form of high performance liquid chromatography. The key difference between HPLC and fast HPLC is that the pump pressure that we use for HPLC is around 40 MPa. Whereas, the pump pressure for fast HPLC is around 3-5 MPa.

What is the difference between HPLC and FPLC?

The HPLC columns are made out of stainless steel. FPLC columns are made out of either glass bodies with high performance resin tops and bottoms or PEEK. Most HPLC resins are made of silica beads with small particle sizes that can stand very high back pressures. FPLC uses agarose, polymer materials, or silica materials.

Why is HPLC better than HPLC?

HPLC methods have many advantages over previously used liquid chromatographic techniques. It allows for higher resolution, better peak shape, reproducible responses and the speed of analysis. ... This allows for better separation than the particle size of 5 μm used in HPLC. It also allows for very fast analysis.

What are the types of HPLC?

Types of HPLC

Why HPLC is called High Performance?

HPLC stands for High Performance Liquid Chromatography. Its earlier name was High Pressure Liquid Chromatography because it involved use of liquid mobile phase requiring higher pressures than gases used in Gas Chromatography.

Why is HPLC used?

High-performance liquid chromatography (HPLC) is a chromatographic technique used to split a mixture of compounds in the fields of analytical chemistry, biochemistry and industrial. The main purposes for using HPLC are for identifying, quantifying and purifying the individual components of the mixture.

What is HPLC principle?

The separation principle of HPLC is based on the distribution of the analyte (sample) between a mobile phase (eluent) and a stationary phase (packing material of the column). ... Hence, different constituents of a sample are eluted at different times. Thereby, the separation of the sample ingredients is achieved.

How accurate is HPLC?

The accuracy calculated in the range of 88–105.9% and the precision (as relative standard deviation) was between 2.7 and 10.9%. These results demonstrate that the developed method can be a fast and accurate method for quantification of silibinin in aqueous samples.

What is UPLC vs HPLC?

A: Both HPLC and UPLC are both liquid chromatography techniques used to separate the different com- ponents found in mixtures. However, UPLC operates at higher pressures (15,000 psi) and allows for lower particle sizes in columns, while HPLC operates at lower pressures (max <6000 psi).

What is difference between TLC and HPLC?

High performance liquid chromatography (HPLC) is a form of column chromatography used to separate compounds with varied polarity. Thin layer chromatography (TLC) is a form of chromatography used to separate compounds with varied polarity, based on a stationary and mobile phases on a silica plate.

Which detector is used in HPLC?

HPLC Detectors

How many types of detectors are there in HPLC?

They are of three types, i.e. fixed wavelength detectors, variable wavelength detectors and the diode array detectors.

Is HPLC quantitative or qualitative?

For most HPLC analyses, peak areas are used for quantitative calculations, although, in most cases, equivalent results may be achieved with peak height.

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